Advanced test-strip technology

Dual-mode electrochemical & fiber-optic test strips:
There are a number of blood glucose meters, such as the Roche Accutrend GCT meter, that also monitor triglycerides. The drawback of these meters is that they require the user to insert a separate (and costly) triglycerides test-strip. This is expensive and inconvenient, and most users won't do this on a routine basis. By contrast, combination electrochemical - fiberoptic strips allow the triglycerides (lipemia) measurement to be performed at the same time as the blood glucose test, with no additional effort or expense. This allows users to continually track their rapidly changing triglyceride levels. This improved monitoring will allow diabetics to get better control over their lipemia, reducing the risk of cardiovascular disease.

Patent, 6,984,307, and its CIP 11/263,500, provide the IP for combination electrochemical - optical test strips that are ideal for type 2 diabetics. Some diagrams of this type of dual test-strip and meter are shown below:

Dual mode test strips and meters

Test-strip with fiber optic turbidity detector Dual blood glucose and lipemia meter

Here near-infrared light from the excitation fiber optic strand penetrates through red cells, but is strongly scattered by triglyceride-rich blood chylomicrons. This scattered light is picked up by the detection fiber optic strand, and is analyzed by a photodetector on the meter. 

Electrochemical immunoassays:

For analytes such as A1c, microalbuminuria, anti-insulin antibodies, c-peptide, and other antigens of interest, immunoassay technology is required. Our electrochemical immunoassay technology (patent 7,166,208) enables simple, high performance, immunoassays be adapted for the well-accepted blood glucose electrochemical test strip format.

Electrochemical immunoassay test strips

Our apoenzyme reactivation immunochemical sensors are similar to conventional blood glucose test-strips, with the exception that the strips use apoglucose oxidase instead of glucose oxidase. The apoglucose oxidase is enzymatically inactive because its cofactor, FAD has been removed. Here the FAD cofactor is conjugated with an antigen, and this antigen is, in-turn, bound to an antibody.

When excess test antigens are added, the test-antigens displace the bound FAD from the antibody. This FAD reactivates apoglucose oxidase. The reactivated glucose oxidase then reacts with glucose (present in the test chemistry), producing an electrochemical signal that is detected by the test strip's electrodes.

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